Lipid peroxidation is a hallmark of many acute and chronic inflammatory conditions. One of its deleterious consequences is the formation of neo-self epitopes, also known as oxidation specific epitopes (OSEs), which act as pro-inflammatory danger signals recognized by innate immunity. Among them, malondialdehyde (MDA)-epitopes are the best-studied example. They are highly enriched on apoptotic cells, oxidized lipoproteins and microvesicles. Together with other OSEs, MDA-epitopes represent endogenous damage-associated molecular patterns (DAMPs) recognized by innate immunity. Based on previous findings, we hypothesized that MDA-epitopes represent important mediators of inflammation and homeostasis maintenance. The aim of this thesis was to define cellular responses of macrophages sensing MDA-epitopes. Furthermore, we studied the presence of MDA-epitopes in non-apoptotic cell death mechanisms. (A) To elucidate MDA-epitope induced migration, cytoskeletal dynamics of bone marrow-derived macrophages (BMDM) were monitored by confocal microscopy. MDA-epitope induced macrophage movement was assessed using scratch and transwell migration assays, in the presence and absence of pharmacological inhibitors as well as an MDA-epitopes neutralizing antibody. (B) Cell death experiments were performed in Jurkat T cells and HT-1080 cells, doing a time-course and dose-dependent stimulation including various inducers and inhibitors of necroptosis and ferroptosis. Visualization and assessment of cell viability and presence of MDA-epitopes on dying cells was performed by flow cytometry and confocal microscopy. Consistent with our hypothesis, MDA-epitopes were shown (1) to induce rapid macrophage spreading with increased filopodia and membrane ruffling, an effect that could be inhibited by the MDA-specific antibody LR04. (2) Secondly, MDA-epitope induced migration was shown to be independent of the scavenger receptors CD36, SR-A1 and TLR4. (3) Furthermore, BMDM migration happened in a dose-dependent manner: While low doses of MDA-epitopes stimulated cell movement, higher amounts of them inhibited migration. (4) The appearance of MDA-epitopes was further demonstrated to coincide with loss of membrane integrity in ferroptosis and necroptosis. All of which contributes to the data supporting the notion that MDA-epitopes represent novel DAMPs, which play an important role in the maintenance of homeostasis.