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Spatial variability and reproducibility of GABA-edited MEGA-LASER 3D-MRSI in the brain at 3T
AuthorBogner, Wolfgang ; Trattnig, Siegfried ; Dobrota, Dusan ; Ukropec, Jozef ; Dydak, Ulrike ; Gajdosik, Martin ; Andronesi, Ovidiu C. ; Strasser, Bernhard ; Povazan, Michal ; Hnilicova, Petra
Published in
NMR in Biomedicine, Hoboken, 2016, Vol. 29, Issue 11, page 1656-1665
PublishedHoboken : Wiley-Blackwell, 2016
Document typeJournal Article
Keywords (EN)acquisition methods / mega editing / mrs and mrsi methods / neurotransmitters / normal brain / reproducibility / magnetic-resonance-spectroscopy / in-vivo detection / real-time motion / mr spectroscopy / regional-variations / glutamate / age / neurotransmitters / schizophrenia / gender
Project-/ReportnumberKLI 61-B00
URNurn:nbn:at:at-ubmuw:3-1974 Persistent Identifier (URN)
 The work is publicly available
Spatial variability and reproducibility of GABA-edited MEGA-LASER 3D-MRSI in the brain at 3T [1.18 mb]
Abstract (English)

The reproducibility of gamma-aminobutyric acid (GABA) quantification results, obtained with MRSI, was determined on a 3T MR scanner in healthy adults. In this study, a spiral-encoded, GABA-edited, MEGA-LASER MRSI sequence with real-time motion-scanner-instability corrections was applied for robust 3D mapping of neurotransmitters in the brain. In particular, the GABA(+) (i.e. GABA plus macromolecule contamination) and Glx (i.e. glutamate plus glutamine contamination) signal was measured. This sequence enables 3D-MRSI with about 3cm(3) nominal resolution in about 20min. Since reliable quantification of GABA is challenging, the spatial distribution of the inter-subject and intra-subject variability of GABA(+) and Glx levels was studied via test-retest assessment in 14 healthy volunteers (seven men-seven women). For both inter-subject and intra-subject repeated measurement sessions a low coefficient of variation (CV) and a high intraclass correlation coefficient (ICC) were found for GABA(+) and Glx ratios across all evaluated voxels (intra-/inter-subject: GABA(+) ratios, CV similar to 8%-ICC>0.75; Glx ratios, CV similar to 6%-ICC>0.70). The same was found in selected brain regions for Glx ratios versus GABA(+) ratios (CV varied from about 5% versus about 8% in occipital and parietal regions, to about 8% versus about 10% in the frontal area, thalamus, and basal ganglia). These results provide evidence that 3D mapping of GABA(+) and Glx using the described methodology provides high reproducibility for application in clinical and neuroscientific studies.

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