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Title
Adenosine to Inosine editing frequency controlled by splicing efficiency
AuthorJantsch, Michael F. ; Mayrhofer, Elisa ; Kapoor, Utkarsh ; Licht, Konstantin
Published in
Nucleic Acids Research, Oxford, 2016, Vol. 44, Issue 13, page 6398
PublishedOxford : Oxford University Press, 2016
LanguageEnglish
Document typeJournal Article
Keywords (EN)pre-messenger-rna / human transcriptome / global regulation / glur-b / receptor / adar2 / sites / gene / identification / deaminase
Project-/ReportnumberP 26845-B20
ISSN0305-1048
URNurn:nbn:at:at-ubmuw:3-1281 Persistent Identifier (URN)
DOI10.1093/nar/gkw325 
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 The work is publicly available
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Adenosine to Inosine editing frequency controlled by splicing efficiency [4.15 mb]
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Abstract (English)

Alternative splicing and adenosine to inosine (A to I) RNA-editing are major factors leading to co- and post-transcriptional modification of genetic information. Both, A to I editing and splicing occur in the nucleus. As editing sites are frequently defined by exon-intron basepairing, mRNA splicing efficiency should affect editing levels. Moreover, splicing rates affect nuclear retention and will therefore also influence the exposure of pre-mRNAs to the editing-competent nuclear environment. Here, we systematically test the influence of splice rates on RNA-editing using reporter genes but also endogenous substrates. We demonstrate for the first time that the extent of editing is controlled by splicing kinetics when editing is guided by intronic elements. In contrast, editing sites that are exclusively defined by exonic structures are almost unaffected by the splicing efficiency of nearby introns. In addition, we show that editing levels in pre- and mature mRNAs do not match. This phenomenon can in part be explained by the editing state of an RNA influencing its splicing rate but also by the binding of the editing enzyme ADAR that interferes with splicing.

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CC-BY-NC-License (4.0)Creative Commons Attribution - NonCommercial 4.0 International License