Go to page
 

Bibliographic Metadata

Title
A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha
AuthorFoisner, Roland ; Dechat, Thomas ; von Haeseler, Arndt ; Skoruppa, Michael P. ; Rescheneder, Philipp ; Gesson, Kevin
Published in
Genome Research, Cold Spring Harbor, 2016, Vol. 26, Issue 4, page 462-473
PublishedCold Spring Harbor : Cold Spring Harbor Laboratory Press, 2016
LanguageEnglish
Document typeJournal Article
Keywords (EN)nuclear-envelope protein / embryonic stem-cells / domain organization / genome interactions / histone methylation / 2-alpha lap2-alpha / chromatin state / prelamin-a / dna / differentiation
Project-/ReportnumberP 26492-B20
ISSN1088-9051
URNurn:nbn:at:at-ubmuw:3-1298 Persistent Identifier (URN)
DOI10.1101/gr.196220.115 
Restriction-Information
 The work is publicly available
Files
A-type lamins bind both hetero- and euchromatin, the latter being regulated by lamina-associated polypeptide 2 alpha [1.84 mb]
Links
Reference
Classification
Abstract (English)

Lamins are components of the peripheral nuclear lamina and interact with heterochromatic genomic regions, termed lamina-associated domains (LADs). In contrast to lamin B1 being primarily present at the nuclear periphery, lamin A/C also localizes throughout the nucleus, where it associates with the chromatin-binding protein lamina-associated polypeptide (LAP) 2 alpha. Here, we show that lamin A/C also interacts with euchromatin, as determined by chromatin immunoprecipitation of euchromatin- and heterochromatin-enriched samples. By way of contrast, lamin B1 was only found associated with heterochromatin. Euchromatic regions occupied by lamin A/C overlap with those bound by LAP2alpha, and lack of LAP2alpha in LAP2alpha-deficient cells shifts binding of lamin A/C toward more heterochromatic regions. These alterations in lamin A/C-chromatin interactions correlate with changes in epigenetic histone marks in euchromatin but do not significantly affect gene expression. Loss of lamin A/C in heterochromatic regions in LAP2alpha-deficient cells, however, correlated with increased gene expression. Our data show a novel role of nucleoplasmic lamin A/C and LAP2alpha in regulating euchromatin.

Stats
The PDF-Document has been downloaded 4 times.
License
CC-BY-License (4.0)Creative Commons Attribution 4.0 International License