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Title
Tropomyosins in mosquito and house dust mite crossreact at the humoral and cellular level
AuthorBohle, Barbara ; Cantillo, Jose F. ; Puerto, Leonardo ; Fernandez-Caldas, Enrique ; Subiza, Jose L. ; Soria, Irene ; Wöhrl, Stefan ; Ebner, Christof ; Keller, Walter ; Resch-Marat, Yvonne ; Vrtala, Susanne
Published in
Clinical & Experimental Allergy, 2018, Vol. 48, Issue 10, page 1354-1363
PublishedWiley-Blackwell, 2018
LanguageEnglish
Document typeJournal Article
Keywords (EN)crossreactivity / house dust mites / mosquito / Tcell epitopes / tropomyosin
URNurn:nbn:at:at-ubmuw:3-396 Persistent Identifier (URN)
DOI10.1111/cea.13229 
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 The work is publicly available
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Tropomyosins in mosquito and house dust mite crossreact at the humoral and cellular level [0.99 mb]
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Abstract (English)

Background

Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including crossreactive tropomyosins. However, the functional and clinical relevance of their crossreactivity is still debated.

Objective

To analyse the humoral and cellular crossreactivity of recombinant Aed a 10.01, Aed a 10.02 and Der p 10.

Methods

Sera from 15 Austrian house dust miteallergic, Der p 10sensitized individuals were tested for IgE reactivity to recombinant tropomyosins in ELISA, inhibition ELISA and basophil activation tests. BALB/c mice were immunized with Aed a 10.01 or Aed a 10.02, and their sera were assessed for reactivity to all tropomyosins. Splenocytes were stimulated with all tropomyosins and synthetic peptides representing the amino acid sequence of Aed a 10.01.

Results

IgE antibodies of Der p 10sensitized patients crossreacted with both tropomyosins from A. aegypti. Aed a 10.01 was a more potent inhibitor of IgE binding to Der p 10 and a stronger activator of basophils sensitized with Der p 10specific IgE than Aed a 10.02. Murine antibodies raised against Aed a 10.01 and Aed a 10.02 crossreacted with Der p 10. Aed a 10.01specific antibody showed stronger crossreactivity with Der p 10 than Aed a 10.02specific antibody. Splenocytes from both groups of mice proliferated similarly to all tropomyosins. Five crossreactive T cellactivating regions were identified.

Conclusion and Clinical relevance

Tropomyosins from D. pteronyssinus and A. aegypti show humoral and cellular crossreactivity, involving 5 potential T cellactivating regions. The more pronounced crossreactivity of Aed a 10.01 and Der p 10 matched the higher sequence similarity of both proteins.

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CC-BY-License (4.0)Creative Commons Attribution 4.0 International License