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Title
Human periodontal ligament cells exhibit no endotoxin tolerance upon stimulation with Porphyromonas gingivalis lipopolysaccharide
AuthorAndrukhov, Oleh ; Blufstein, A. ; Behm, C. ; Nguyen, P.Q. ; Rausch-Fan, X.
Published in
Journal of Periodontal Research, 2018, Vol. 53, Issue 4, page 589-597
PublishedWiley-Blackwell, 2018
LanguageEnglish
Document typeJournal Article
Keywords (EN)endotoxin tolerance / lipopolysaccharide / periodontal ligament cells / periodontitis / Porphyromonas gingivalis
URNurn:nbn:at:at-ubmuw:3-641 Persistent Identifier (URN)
DOI10.1111/jre.12549 
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Human periodontal ligament cells exhibit no endotoxin tolerance upon stimulation with Porphyromonas gingivalis lipopolysaccharide [0.79 mb]
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Abstract (English)

Background/Objectives

Endotoxin tolerance is characterized by a state of hyporesponsiveness after confrontation with endotoxins such as lipopolysaccharides (LPS) at low concentrations. The aim of this study was to investigate, whether pretreatment with Porphyromonas gingivalis leads to endotoxin tolerance induction and possible alterations in tolllike receptor (TLR) 2 and 4induced response in human periodontal ligament cells (hPDLCs).

Material and Methods

Primary hPDLCs were pretreated with P. gingivalis (0.1 or 0.3 g/mL) LPS for 24 hours and afterwards treated with one of the following stimuli: P. gingivalis LPS (1 g/mL); TLR4 agonist Escherichia coli LPS (0.1 g/mL; 1 g/mL); TLR2 agonist Pam3CSK4 (0.1 g/mL; 1 g/mL). The protein expression of interleukin (IL)6, IL8 and monocyte chemotactic protein1 was analyzed with quantitative polymerase chain reaction and enzymelinked immunosorbent assay. Gene expression levels of TLR2 and TLR4 were determined by quantitative polymerase chain reaction.

Results

Pretreatment of cells with low concentrations of P. gingivalis LPS did not result in lower production of IL6, IL8 and monocyte chemotactic protein1 compared to control group. In some cases, pretreated cells exhibited lower gene expression levels of TLR2 and TLR4 compared to nonpretreated cells.

Conclusion

The results of this study implicate that hPDLCs do not develop endotoxin tolerance. Furthermore, the amplitude of the inflammatory response shows no significant dependency on TLR2 and TLR4 expression levels.

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CC-BY-License (4.0)Creative Commons Attribution 4.0 International License