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Title
Absolute Quantification of Phosphor-Containing Metabolites in the Liver Using 31P MRSI and Hepatic Lipid Volume Correction at 7T Suggests No Dependence on Body Mass Index or Age
AuthorKrssak, Martin ; Pfleger, Lorenz ; Gajdosik, Martin ; Wolf, Peter ; Smajis, Sabina ; Fellinger, Paul ; Kuehne, Andre ; Krumpolec, Patrik ; Trattnig, Siegfried ; Winhofer, Yvonne ; Krebs, Michael ; Chmelik, Marek
Published in
Journal of Magnetic Resonance Imaging, 2019, Vol. 49, Issue 2, page 597-607
PublishedWiley-Blackwell, 2019
LanguageEnglish
Document typeJournal Article
Keywords (EN)Keywords / phosphorus / magnetic resonance spectroscopic imaging / 7T / absolute quantification / liver
URNurn:nbn:at:at-ubmuw:3-830 Persistent Identifier (URN)
DOI10.1002/jmri.26225 
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Absolute Quantification of Phosphor-Containing Metabolites in the Liver Using 31P MRSI and Hepatic Lipid Volume Correction at 7T Suggests No Dependence on Body Mass Index or Age [28.04 mb]
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Abstract (English)

Background

Hepatic disorders are often associated with changes in the concentration of phosphorus31 (31P) metabolites. Absolute quantification offers a way to assess those metabolites directly but introduces obstacles, especially at higher field strengths (B0 7T).

Purpose

To introduce a feasible method for in vivo absolute quantification of hepatic 31P metabolites and assess its clinical value by probing differences related to volunteers' age and body mass index (BMI).

Study Type

Prospective cohort.

Subjects/Phantoms

Four healthy volunteers included in the reproducibility study and 19 healthy subjects arranged into three subgroups according to BMI and age. Phantoms containing 31P solution for correction and validation.

Field Strength/Sequence

Phaseencoded 3D pulseacquire chemical shift imaging for 31P and singlevolume 1H spectroscopy to assess the hepatocellular lipid content at 7T.

Assessment

A phantom replacement method was used. Spectra located in the liver with sufficient signaltonoise ratio and no contamination from muscle tissue, were used to calculate following metabolite concentrations: adenosine triphosphates ( and ATP); glycerophosphocholine (GPC); glycerophosphoethanolamine (GPE); inorganic phosphate (Pi); phosphocholine (PC); phosphoethanolamine (PE); uridine diphosphateglucose (UDPG); nicotinamide adenine dinucleotidephosphate (NADH); and phosphatidylcholine (PtdC). Correction for hepatic lipid volume fraction (HLVF) was performed.

Statistical Tests

Differences assessed by analysis of variance with Bonferroni correction for multiple comparison and with a Student's ttest when appropriate.

Results

The concentrations for the young lean group corrected for HLVF were 2.56 0.10 mM for ATP (mean standard deviation), ATP: 2.42 0.15 mM, GPC: 3.31 0.27 mM, GPE: 3.38 0.87 mM, Pi: 1.42 0.20 mM, PC: 1.47 0.24 mM, PE: 1.61 0.20 mM, UDPG: 0.74 0.17 mM, NADH: 1.21 0.38 mM, and PtdC: 0.43 0.10 mM. Differences found in ATP levels between lean and overweight volunteers vanished after HLVF correction.

Data Conclusion

Exploiting the excellent spectral resolution at 7T and using the phantom replacement method, we were able to quantify up to 10 31Pcontaining hepatic metabolites. The combination of 31P magnetic resonance spectroscopy imaging data acquisition and HLVF correction was not able to show a possible dependence of 31P metabolite concentrations on BMI or age, in the small healthy population used in this study.

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CC-BY-NC-License (4.0)Creative Commons Attribution - NonCommercial 4.0 International License